Biography
Maria LuÃsa Serralheiro has completed her PhD from Lisbon University. She is Assistant Professor with Habilitation, at the Department of Chemistry and Biochemistry. She teaches Chemistry and Biochemistry and also does research in the area of Applied Biochemistry for 35 years. She has published more than 55 papers in reputed journals and has been supervising more than 24 Master Thesis and PhD Thesis. She is responsible for several disciplines in Chemistry and Biochemistry master Course as well as in the Biochemistry undergraduate program. She received a prize from «Food & Nutrition Award» in 2014.
Abstract
Aqueous extracts from plants have been consumed throughout history for the prevention or amelioration of several diseases namely, atherosclerosis and Alzheimer. Nowadays part of the molecular mechanisms underlying these effects have been disclosed. The effect of infusions containing mainly rosmarinic acid, chlorogenic acid and other caffeic acid derivatives were analysed on what concerns their effect on the prevention of age-related diseases like atherosclerosis and Alzheimer disease (AD). As these diseases are multifactorial-caused the main selected topics will be the inhibitory activity of these compounds or extracts on enzymes involved in cholesterol biosynthesis (3-hydroxy-3-methylglutaryl-CoA reductase, HMGR) and in the AD treatment (acetylcholinesterase, AChE). The effect of these caffeic acid derivatives on the absorption of cholesterol from the diet at membrane-transporter proteins is also a matter of concern and will be envisaged. Several infusions from different herbs either collected in the fields or bought in the supermarket, in Portugal, were studied. A small number of volunteers consumed several of these infusions in order to get some insight into the effect in decreasing cholesterol blood level and, eventually, reducing atherosclerosis. The results obtained seemed worthwhile of setting up a bigger experimentation plan. The permeation of phenolic compounds has been evaluated and studies with laboratory animals indicated that these compounds can reach the brain and act as AChE inhibitors. The presence of different phenolic compounds in these mixtures influence the permeation of each other. This is also another important point to be addressed, paving the way to permeation studies with chemically defined extracts when bioavailability is concerned.
Biography
Emre Kayalar has completed his PhD from Istanbul University, Dentistry Facuty, Department of Orthodontics in 2014. He began postdoctoral studies and studying for second masters degree in Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Botany. He has published more than 5 papers in national or international journals. He participated in many national and international congresses with some presentations.
Abstract
Paliurus spina-christi P. Mill. (Rhamnaceae), also known as “Christ\'s thorn, Karaçalı or Çaltıâ€, is a perennial thorny shrub of widespread distribution in dry and rocky places in the Mediterranean region and Asia, and the unique species of the genus Paliurus represented in Anatolia. Christ\'s thorn is used as a diuretic, against diarrhoea and rheumatism in traditional Turkish herbal medicine. The aim of this study was to determine the antioxidant activity and phenolic profile of Paliurus spina-christi. For this purpose plant materials were collected from EskiÅŸehir:BozdaÄŸ in Turkey on August 2015. Powdered dried fruits were macerated with methanol at 25 °C for 24h. After evaporation and lyofilization steps the extract was analyzed with ABsciex 3200 Q trap LC-MS/MS system. Catechin hydrate, proanthocyanidin B dimer, naringenin-C-diglycoside, 5-caffeoyl quinic acid 5-p-coumaroylquinic acid, rutin, quercetin hexoside and quercetin were found as major compounds. Extract presented strong antioxidant activity with the score of DPPH IC50=0.062 mg/ml. IC 50 of BHT was determined 0,054 mg/ml. Total phenolic content of the extract was found as same 109.54 mgGAE in 1gr extract. This study was supported by the Anadolu University, Commission of the Scientific Research Projects.