Nayan G Patel
Dharmsinh Desai University, India
Title: Validated HPTLC method for quantification of luteolin and apigenin in Premna mucronata Roxb., Verbenaceae
Biography
Biography: Nayan G Patel
Abstract
A simple, rapid and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin and apigenin in Premna mucronata Roxb., family Verbenaceae. Separation was performed on silica gel 60 F254 HPTLC plates using Toluene: Ethyl acetate: Formic acid (6:4:0.3) as mobile phase for elution of markers from extract. The determination was carried out in fluorescence mode using densitometric absorbance-reflection mode at 366 nm for both luteolin and apigenin. The methanolic extract of Premna mucronata was found to contain 10.2 mg/g% luteolin and 0.165 mg/g% of apigenin. The method was validated in terms of linearity, LOD and LOQ, accuracy, precision and specificity. The calibration curve was found to be linear between 200-1000 ng/band for luteolin and 50-250 ng/band for apigenin. For luteolin and apigenin, the limit of detection was found to be 508.13 ng/band and 7.97 ng/band while the limit of quantitation was found to be 1539.78 ng/band and 24.155 ng/band, respectively. This developed validated method is capable of quantifying and resolving luteolin and apigenin in its plant raw-material.