Ahmed Abdelfatah Abdallah sedik
Nationl Research Center,Egypt
Title: Trigonelline prevents hepatic oxidative stress, lipid peroxidation and molecular alterations in insulin resistant rats
Biography
Biography: Ahmed Abdelfatah Abdallah sedik
Abstract
The objective of the present study was to evaluate the effect of trigonelline (TRIG) on the hepatic complications associated with in high fat high fructose-induced insulin resistance in rats. Insulin resistance was induced by adding high-fat diet (60 kcal/100 kcal saturated fat) and 10% fructose in the drinking water to rats for 8 weeks. Insulin resistant rats were orally treated with either TRIG (50 and 100 mg/kg), sitagliptin (SITA; 10 mg/kg) or the combination TRIG (50 mg/kg) with SITA (10 mg/kg) for 14 consecutive days. Twenty-four hours after the last dose of the drugs, the 18 hours fasting rats were weighed; blood, liver and pancreas samples were isolated. Sera were separated for determination of serum levels of glucose and insulin; for calculating the homeostatic model assessment–insulin resistance (HOMA-IR). Liver homogenates were used for assessment of hepatic oxidative stress biomarkers and inflammatory cytokines.. Moreover, hepatic tissues were examined using Fourier Transform Infrared (FTIR) micro-spectroscopy technique for assessment of any molecular changes. Results of the present study revealed that HFHF treated rats was associated with a marked increase in the fasting serum levels of glucose and insulin thus, an increased HOMA-IR. Insulin resistant rats was also associated with a marked increase in the hepatic oxidative stress biomarkers; measured as malondialdehyde (MDA) and reduced glutathione (GSH) and the inflammatory cytokine; α-tumor necrosis factor (α-TNF). Oral treatment of insulin resistant rats with TRIG and its combination with SITA significantly decreased HOMA-IR, hepatic MDA, GSH and α-TNF. TRIG and its combination with SITA succeeded to ameliorate the histopathological, cytometery and molecular alteration induced by HFHF. From all the previous results, it can be concluded that TRIG has a beneficial effect on the hepatic complications associated with HFHF-induced insulin resistance in rats due to its hypoglycemic effect and antioxidant potential.