Rodolfo de Moraes Peixoto
Science and Technology of the Sertão Pernambucano, Brazil
Title: Treatment of goat mastitis experimentally induced by Staphylococcus aureus using a formulation containing Hymenaea martiana extract
Biography
Biography: Rodolfo de Moraes Peixoto
Abstract
The aim of the present study was to assess the in vivo potential of therapy based on Hymenaea martiana Hayne extract in mastitis cases among goats that were experimentally induced with strains of Staphylococcus aureus. The phytochemical characterization of the plant extract was performed using HPLC-DAD. Ten female goats were experimentally infected with a clinical strain of S. aureus. One group was treated with a commercial antimicrobial and another with ointment from natural plant extract. The diagnosis of mastitis was performed using a bacterial culture, SCC, CFU/mL and CMT. Three milk samples were subjected to chromatography to survey the constituents present in the ointment prepared with the plant extract. The results of the HPLC confirmed the presence of phenolic compounds in the chloroform and ethyl acetate. Concerning SCC and CFU/ml, the analysis of variance performed between the experimental periods confirmed that there was a higher score at the time of the first collection after infection in all of the experimental groups. After treatment with ointment containing the extract of H. martiana and commercial antimicrobials, these variables returned to values indicative of an absence of intra-mammary infection, based on the international standards for raw goat’s milk. In the present study, milk collected on the 32nd day post treatment with ointment containing the extract of H. martiana exhibited a significant reduction in CFU/ML, when compared with the control group. Antibacterial activity may be related to the classes of secondary metabolites found.