Gholamali Jelodar
Shiraz University, Iran
Title: The Cyclohexane Extract of Walnut Leaf Improves Indices of Oxidative Stress, TotalHomocysteineand Lipids Profiles in Streptozotocin Induced Diabetic Rats
Biography
Biography: Gholamali Jelodar
Abstract
Mechanism of most herbal drugs used for diabetes mellitus treatment has not been well defined. The aim of this study was to evaluate the effect of two doses of cyclohexanic extract of walnut leaf onhomocysteine, lipids profil (total cholesterol, TG, HDL-c, LDL-c, VLDL) and indices of oxidative stress (SOD, GPx, CAT, MDA) in diabetic rats. Twenty-eight male Sprague Dawley rats were randomly divided into four groups, group I: control (received sesame oil as vehicle), group II: diabetic (received sesame oil), group III and IV: diabetic treated by 150 and 250 mg/kg bodyweight (BW) per day extract of walnut leaf, respectively. All groups were treated for 28 days via oral gavage. Diabetes was induced by single IP injection of streptozotocin (50 mg/kg BW). Fasting blood glucose (FBG) level and body weight were measured before injection, 3 days after injection, and on days 0, 7, 14, 21 and 28 of treatment. At the end of day 28, blood samples were collected via heart puncture and the sera were used for estimation of the above mentioned parameters. The results showed decrease in glucose level, TC, TG, LDL-c, VLDL-c, homocysteine, and MDA level and increase in the level of HDL-c in a dose-dependent manner at the end of 28 days (P<0.05). The activity of antioxidant enzymes increased in treated group compared with diabetic control (P<0.05). The results demonstrate that cyclohexane extract of walnut leaf has an overall beneficial effect on body weight, fasting blood glucose, lipids profile, antioxidant enzymes activity and homocysteine in an experimental model.