Laila Jafri
Quaid-i-Azam University, Pakistan
Title: Newer strategies in herbal drug research and development: focus on reverse pharmacology
Biography
Biography: Laila Jafri
Abstract
Glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are incretin hormones that potentiate insulin secretion in a glucose-dependent manner. Selective GLP-1 and GIP secretagogue are in development as novel treatments for type 2 diabetes. Here, recent reports indicate that H. nepalensis (crude extract and fractions) and its isolated compound lupeol stimulates secretion of GLP-1 and GIP in pGIP/neo STC-1cells. Lupeol stimulated GLP-1 and GIP secretion from STC-1 cells in a concentration-dependent manner. Futhermore, proglucagon, GIP and prohormone convertase 3 genes regulating GLP-1 and GIP biosynthesis were analyzed by RT-PCR. Lupeol also promoted proglucagon, GIP and prohormone convertase 3 mRNA expression. The present results first time demonstrated that H. nepalensis (crude extract and fractions) and its isolated compound lupeol showed its modulation on both incretine hormones via promoting their secretion and biosynthesis.