Yu Zepu
Yunnan University of Traditional Chinese Medicine, China
Title: Effect of Yajieshaba, a preparation of Dai indigenous medicine, on enhanced liver detoxification
Biography
Biography: Yu Zepu
Abstract
Yajieshaba(YJSB), prepared by Xishuangbanna Hospital of Dai Indigenous Medicine(Xishuangbanna, China), has a long history of clinical use. YJSB is commonly used in clinical detoxification by Dai doctors, but the detoxification mechanism is not clear. Therefore, the purpose of our study was to explore the mechanistic effects of Yajieshaba(YJSB) on enhanced liver detoxification, which provide the pharmacological experimental basis for clinically safe use. The effects of YJSB on alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assayed in five acute chemical liver injury models [ carbon tetrachloride (CCL4), D-galactorsamine(D-Glan), 4-acetamidophenol (AAP), thioacetamide (TAA) and 1-naphthyl isothiocyanate(ANIT)]. Sleep latency and sleep time of pentobarbital sodium were tested in control mice and CCl4 model mice after oral YJSB administration. The effects of YJSB on drug metabolism enzymes of liver microsomes were tested in control rats and CCl4 model rats. The levels of cytochrome P450 (CYP450) and Cyt b5 in liver microsomes were assyed using the method by Omura and Sato, and activities of erythromycin N-demethylase (ERD) and aminopyrine N-demethyl (ADM) were evaluated by Nash colorimetry. Probe substrate-based high performance liquid chromatography (HPLC) methods were established for CYP3A4 and CYP1A2. The results showed that the level of serum ALT was reduced by YJSB at 3.51g/kg in the five models as follows: CCl4>D-Glan, AAP, ANIT>TAA. YJSB treatment did not reduce the level of serum AST. YJSB at 3.51g/kg prolonged the sleep latency in control mice and shortened the sleep time of control mice and CCl4 model mice. For control rats, YJSB at 2.43g/kg increased the levels of CYP450 and Cyt b5 and induced the activities of ERD and ADM; for liver injuries induced by CCl4 in rats, YJSB at 2.43g/kg increase the levels of CYP450 and Cyt b5. These results suggest that YJSB at 2.43 g/kg induces CYP3A4 and CYP1A2. In summary, these results demonstrate that YJSB enhances liver detoxification and the mechanisms may be partially related to CYP3A4 and CYP1A2 induction.