Pharmacology and Ethnopharmacology

Biography

Biography: Emerson Silva Lima

Abstract

Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species named “breu”. Gum and oil-resins of this species are used by local population as a tonic and stimulant, as well as for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpenes compounds isolated from oil-resin of Protium paniculatum. The pentacyclic triterpenes αβ-amyrin, αβ-amyrin acetylated, αβ-amyrone and brein/maniladiol did not alter the viability of murine J774 macrophages (IC50 > 20 µg/mL), with the exception of mixture brein/maniladiol which showed moderate cytotoxic activity. Also it was observed that compounds at 10 µg/mL inhibited more than 80% production of NO•, although only αβ-amyrin was able to inhibit the production of TNF-α (52.03 ± 2.4%). The compounds inhibited the production of IL-6 and induced the production of IL-10 in murine J774 macrophages stimulated by LPS. αβ-amyrone inhibited the expression of COX-2 and also inhibited the formation of paw or ear edema in rats and mice, having a quick and immediate effect. This study may provide the basis for future investigations on the therapeutic role of αβ-amyrone in treating inflammation.